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>   首页   >   产品   >   一抗   >   癌症   >   EGFR Antibody (Y1092)   

EGFR Antibody (Y1092)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - EGFR Antibody (Y1092) AW5174
    Western blot analysis of lysates from Hela,PC-3 cell line (from left to right), using Phospho-EGFR-pY1092.ctrl(Cat. #AW5174). AW5174 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 1 - EGFR Antibody (Y1092) AW5174
    "All lanes : Anti-EGFR Antibody at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: PC-3 whole cell lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST."
  • 14 - EGFR Antibody (Y1092) AW5174
    Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (Y1092)(Cat#AW5174). AW5174 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P00533
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=134;M=135;Rat=137 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 1956
Antigen Region 1070-1099
Other Names EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1070-1099 amino acids from human EGFR.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGFR Antibody (Y1092) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EGFR
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Research Areas

BACKGROUND

The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

REFERENCES

Zanardi, T.A., et al., J. Virol. 77(21):11685-11696 (2003).
Krug, A.W., et al., J. Biol. Chem. 278(44):43060-43066 (2003).
Huang, F., et al., J. Biol. Chem. 278(44):43411-43417 (2003).
He, Y.Y., et al., J. Biol. Chem. 278(43):42457-42465 (2003).
Hirsch, F.R., et al., J. Clin. Oncol. 21(20):3798-3807 (2003).

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