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IDH1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - IDH1 Antibody (Center) AW5173
    Western blot analysis of lysates from HepG2,MCF-7 cell line and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)(Cat. #AW5173). AW5173 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 3 - IDH1 Antibody (Center) AW5173
    Confocal immunofluorescent analysis of IDH1 Antibody (Center)(Cat#AW5173) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 14 - IDH1 Antibody (Center) AW5173
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - IDH1 Antibody (Center) AW5173
    IDH1 Antibody (Center) (Cat. #AW5173) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 14 - IDH1 Antibody (Center) AW5173
    Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center)(Cat#AW5173). AW5173 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, IF, WB
Primary Accession O75874
Other Accession P41562, O88844, Q9XSG3
Reactivity Human, Rat
Predicted Mouse, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=47;M=47;Rat=47 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 3417
Antigen Region 116-143
Other Names IDH1; PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:100
FC~~1:10~50
Target/Specificity This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIDH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDH1
Synonyms PICD
Cellular Location Cytoplasm. Peroxisome
Research Areas

BACKGROUND

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

REFERENCES

Geisbrecht B.V., Gould S.J.J. Biol. Chem. 274:30527-30533(1999)
Xu X., Zhao J., Xu Z.J. Biol. Chem. 279:33946-33957(2004)
Bleeker F.E., Lamba S.Hum. Mutat. 30:7-11(2009)

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