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>   首页   >   产品   >   一抗   >   癌症   >   SPHK1 Antibody (N-term P74)   

SPHK1 Antibody (N-term P74)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SPHK1 Antibody (N-term P74) AW5172
    Western blot analysis of lysates from HepG2 cell line and human heart,skeletal muscle,kidney tissue lysate(from left to right),using SPHK1 Antibody (N-term P74)(Cat. #AW5172). AW5172 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • 14 - SPHK1 Antibody (N-term P74) AW5172
    Immunohistochemical analysis of paraffin-embedded H. kidney section using SPHK1 Antibody(N-term P74) (Cat#AW5172). AW5172 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - SPHK1 Antibody (N-term P74) AW5172
    Immunohistochemical analysis of paraffin-embedded M. kidney section using SPHK1 Antibody(N-term P74) (Cat#AW5172). AW5172 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession Q9NYA1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=43,51 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 8877
Antigen Region 59-89
Other Names SPHK1; SPHK; SPK; Sphingosine kinase 1
Dilution WB~~1:1000
IHC-P~~1:100
Target/Specificity This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SPHK1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPHK1 Antibody (N-term P74) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK1
Synonyms SPHK, SPK
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Cellular Location Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner
Tissue Location Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle
Research Areas

BACKGROUND

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

REFERENCES

Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Nava, V.E., et al., FEBS Lett. 473(1):81-84 (2000).
Melendez, A.J., et al., Gene 251(1):19-26 (2000).
Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000).

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