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PGK1 Antibody (Center S320)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PGK1 Antibody (Center S320) AW5171
    Western blot analysis of lysates from Hela,A431,mouse Ba/F3 cell line (from left to right), using PGK1 Antibody (S320)(Cat. #AW5171). AW5171 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - PGK1 Antibody (Center S320) AW5171
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 Antibody (Center S320)(Cat.#AW5171), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P00558
Other Accession Q60HD8, Q5J7W1
Reactivity Human, Mouse
Predicted Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=45;M=45;Rat=45 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5230
Antigen Region 305-334
Other Names PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2
Dilution WB~~1:1000
IHC-P~~1:10~50
Target/Specificity This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human PGK1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGK1 Antibody (Center S320) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGK1
Synonyms PGKA
Function In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.

REFERENCES

Shetty, S., et al., Am. J. Respir. Cell Mol. Biol. 31(1):100-106 (2004).
Daly, E.B., et al., Biochim. Biophys. Acta 1691(1):17-22 (2004).
Daly, E.B., et al., Int. J. Biol. Markers 19(2):170-172 (2004).
Saito, Y., et al., Biochem. Biophys. Res. Commun. 314(2):396-402 (2004).
Krishnan, P., et al., J. Biol. Chem. 278(38):36726-36732 (2003).

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