|Application ||IF, IHC-P, FC, WB|
|Calculated MW||H=48,54,49,53;M=48;Rat=48 KDa|
|Other Names||PDIA6; ERP5; P5; TXNDC7; Protein disulfide-isomerase A6; Endoplasmic reticulum protein 5; Protein disulfide isomerase P5; Thioredoxin domain-containing protein 7|
|Target/Specificity||This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDIA6 Antibody (Center K159) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ERP5, P5, TXNDC7|
|Function||May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed:12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed:24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed:24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed:15466936).|
|Cellular Location||Endoplasmic reticulum lumen. Cell membrane. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545)|
|Tissue Location||Expressed in platelets (at protein level).|
Provided below are standard protocols that you may find useful for product applications.
Protein disulfide isomerases (EC 18.104.22.168), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.
Hayano,T.,Gene 164 (2), 377-378 (1995)