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SFN Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SFN Antibody (C-term) AW5163
    Western blot analysis of lysates from A431,Hela cell line (from left to right), using SFN Antibody (C-term)(Cat. #AW5163). AW5163 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - SFN Antibody (C-term) AW5163
    SFN antibody (C-term) (Cat. #AW5163) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - SFN Antibody (C-term) AW5163
    Confocal immunofluorescent analysis of SFN Antibody (C-term)(Cat. #AW5163) with A549 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 4 - SFN Antibody (C-term) AW5163
    SFN Antibody (C-term) (Cat. #AW5163) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IF, IHC-P
Primary Accession P31947
Other Accession NP_006133.1
Reactivity Human
Predicted Mouse, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB27523
Calculated MW H=28,24;M=28 KDa
Additional info
Gene ID 2810
Other Names SFN; HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin
Target/Specificity This SFN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-248 amino acids from the C-terminal region of human SFN.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSFN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SFN
Synonyms HME1
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
Cellular Location Cytoplasm. Nucleus. Secreted. Note=May be secreted by a non-classical secretory pathway
Tissue Location Present mainly in tissues enriched in stratified squamous keratinizing epithelium
Research Areas
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