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Phospho-SHP2(Y546) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Phospho-SHP2(Y546) Antibody AW5158
    Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-SHP2-Y546 Antibody(EV)(upper) or Beta-actin (lower).
  • 1 - Phospho-SHP2(Y546) Antibody AW5158
    Western blot analysis of lysate from NIH/3T3 cell line, using Phospho-SHP2-Y546 Antibody(Cat. #AW5158). AW5158 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysate at 20ug.
  • 6 - Phospho-SHP2(Y546) Antibody AW5158
    Dot blot analysis of anti-Phospho-SHP2-Y546 Phospho-specific Pab (Cat. #AW5158 ) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, WB
Primary Accession Q06124
Other Accession P41499, P35235, Q90687
Reactivity Human, Mouse
Predicted Rat, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=68 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5781
Antigen Region 521-551
Other Names PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3
Dilution WB~~1:1000
DB~~1:500
Target/Specificity This SHP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y546 of human SHP2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-SHP2(Y546) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPN11
Synonyms PTP2C, SHPTP2
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.
Cellular Location Cytoplasm.
Tissue Location Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Research Areas

BACKGROUND

SHP2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

REFERENCES

Nystrom,A.M., et.al., Eur J Med Genet (2010) In press
Carver,K.C., et.al., J. Biol. Chem. 285 (11), 8003-8012 (2010)

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