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MDH2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - MDH2 Antibody (Center) AW5150
    Western blot analysis of lysates from A431,Hela,U-87 MG,NIH/3T3 cell line (from left to right), using MDH2 Antibody (Center)(Cat. #AW5150). AW5150 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - MDH2 Antibody (Center) AW5150
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - MDH2 Antibody (Center) AW5150
    Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P40926
Other Accession P04636, P00346, P08249, Q4R568, O02640, Q32LG3, P22133
Reactivity Human, Mouse
Predicted Rat, Monkey, Bovine, C.Elegans
Host Rabbit
Clonality Polyclonal
Calculated MW H=36;M=36;Rat=36 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4191
Antigen Region 86-115
Other Names MDH2; Malate dehydrogenase, mitochondrial
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Target/Specificity This MDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the Central region of human MDH2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMDH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MDH2
Cellular Location Mitochondrion matrix.
Research Areas

BACKGROUND

MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

REFERENCES

Rzem,R.,et.al., J. Inherit. Metab. Dis. 30 (5), 681-689 (2007)

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