注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   信号转导   >   HSPD1 Antibody (Center)   

HSPD1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - HSPD1 Antibody (Center) AW5147
    Western blot analysis of lysates from A431,mouse NIH/3T3,rat PC-12 cell line (from left to right), using HSPD1 Antibody (Center)(Cat. #AW5147). AW5147 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 3 - HSPD1 Antibody (Center) AW5147
    Fluorescent image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2859C HSPD1 (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000144381).
  • 3 - HSPD1 Antibody (Center) AW5147
    Fluorescent confocal image of Hela cell stained with HSPD1 Antibody (Center)(Cat#AW5147). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly.
  • 产品详情
  • 实验流程
  • 背景知识
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB
Primary Accession P10809
Other Accession P63039, P63038, P18687, Q5ZL72, P31081
Reactivity Human, Mouse
Predicted Rat, Hamster, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=61;M=61;Rat= 61,27 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 3329
Antigen Region 187-215
Other Names HSPD1; HSP60; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; Heat shock protein 60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Dilution WB~~1:1000
IF~~1:10~50
Target/Specificity This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-215 amino acids from the Central region of human HSPD1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPD1
Synonyms HSP60
Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Cellular Location Mitochondrion matrix.
Research Areas

BACKGROUND

HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.

REFERENCES

References for protein:
1.Venner T.J., Singh B., Gupta R.S.DNA Cell Biol. 9:545-552(1990)
2.Hansen J.J., Bross P., Westergaard M., Nielsen M.N., Eiberg H.,Hum. Genet. 112:71-77(2003)
3.Rasmussen R.K., Ji H., Eddes J.S., Moritz R.L.,Electrophoresis 18:588-598(1997)
4.Aboulaich N., Vainonen J.P., Stralfors P., Vener A.V.Biochem. J. 383:237-248(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69369083 tech.abgent@wuxiapptec.com.


我要评价