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UCHL1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - UCHL1 Antibody (C-term) AW5142
    Western blot analysis of lysates from SH-SY5Y cell line,mouse brain tissue lysate (from left to right), using UCHL1 Antibody (C-term)(Cat. #AW5142). AW5142 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 1 - UCHL1 Antibody (C-term) AW5142
    Western blot analysis of lysates from mouse Neuro-2a,U266,rat PC-12 cell line ,mouse brain tissue lysate(from left to right), using UCHL1 Antibody (C-term)(Cat. #AW5142). AW5142 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 1 - UCHL1 Antibody (C-term) AW5142
    Western blot analysis of lysates from U266,PC-12,NCI-H1299 cell line and mouse brain tissue lysate(from left to right), using UCHL1 Antibody (C-term)(Cat. #AW5142). AW5142 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P09936
Other Accession Q00981, Q6SEG5, Q9R0P9, Q60HC8, P23356
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB21220
Calculated MW H=25;M=25;Rat=25 KDa
Additional info
Gene ID 7345
Other Names UCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1
Target/Specificity This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUCHL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UCHL1
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Cellular Location Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain
Tissue Location Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients
Research Areas

BACKGROUND

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).

REFERENCES

Maraganore, D.M., et al., Mov Disord 18(6):631-636 (2003).
Nishikawa, K., et al., Biochem. Biophys. Res. Commun. 304(1):176-183 (2003).
Liu, Y., et al., Cell 111(2):209-218 (2002).
Caballero, O.L., et al., Oncogene 21(19):3003-3010 (2002).
Saigoh, K., et al., Nat. Genet. 23(1):47-51 (1999).

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