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C21orf33 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - C21orf33 Antibody (Center) AW5137
    Western blot analysis of lysates from Hela, HepG2, K562 cell line (from left to right), using C21orf33 Antibody (Center)(Cat. #AW5137). AW5137 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 3 - C21orf33 Antibody (Center) AW5137
    Fluorescent image of MCF-7 cells stained with C21orf33 Antibody (Center)(Cat#AW5137). AW5137 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF
Primary Accession P30042
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Clone Names RB49805
Calculated MW H=28,25;M=28;Rat=28 KDa
Additional info
Gene ID 102724023;8209
Other Names ES1 protein homolog, mitochondrial, Protein GT335, Protein KNP-I, C21orf33, HES1, KNPI
Target/Specificity This C21orf33 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-124 amino acids from the Central region of human C21orf33.
Dilution WB~~ 1:1000
IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC21orf33 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name C21orf33
Synonyms HES1, KNPI
Cellular Location Mitochondrion.
Tissue Location Ubiquitous, but strongly expressed in heart and skeletal muscle

REFERENCES

Nagamine K.,et al.Biochem. Biophys. Res. Commun. 225:608-616(1996).
Lafreniere R.G.,et al.Genomics 38:264-272(1996).
Scott H.S.,et al.Hum. Genet. 99:616-623(1997).
Hattori M.,et al.Nature 405:311-319(2000).
Nagamine K.,et al.Genomics 42:528-531(1997).

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