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DFFB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DFFB Antibody (Center) AW5135
    Western blot analysis of lysates from A431,Hela cell line (from left to right), using DFFB Antibody (Center)(Cat. #AW5135). AW5135 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - DFFB Antibody (Center) AW5135
    Flow cytometric analysis of Hela cells using DFFB Antibody (Center)(green, Cat#AW5135) compared to an isotype control of rabbit IgG(blue). AW5135 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - DFFB Antibody (Center) AW5135
    Immunohistochemical analysis of paraffin-embedded H.kidney section using DFFB Antibody (Center)(Cat#AW5135). AW5135 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - DFFB Antibody (Center) AW5135
    Immunohistochemical analysis of paraffin-embedded M.kidney section using DFFB Antibody (Center)(Cat#AW5135). AW5135 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession O76075
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW H=39,31;M=39;Rat=40 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 1677
Antigen Region 203-238
Other Names DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40
Dilution WB~~1:1000
FC~~1:25
IHC-P~~1:25
Target/Specificity This DFFB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from the Central region of human DFFB.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDFFB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DFFB
Synonyms CAD, DFF2, DFF40
Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Cellular Location Cytoplasm. Nucleus.
Research Areas

BACKGROUND

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

REFERENCES

Liu X.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8461-8466(1998).
Mukae N.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:9123-9128(1998).
Halenbeck R.,et al.Curr. Biol. 8:537-540(1998).
Nakagawara A.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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