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NDUFC2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NDUFC2 Antibody (N-term) AW5133
    Western blot analysis of lysates from Hela cell line,human kidney tissue,HepG2,U-87 MG,U-251 MG cell line (from left to right), using NDUFC2 Antibody (N-term)(Cat. #AW5133). AW5133 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - NDUFC2 Antibody (N-term) AW5133
    Flow cytometric analysis of HepG2 cells using NDUFC2 Antibody (N-term)(green, Cat#AW5133) compared to an isotype control of rabbit IgG(blue). AW5133 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - NDUFC2 Antibody (N-term) AW5133
    Fluorescent image of HepG2 cells stained with NDUFC2 Antibody (N-term) (Cat#AW5133). AW5133 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
  • 14 - NDUFC2 Antibody (N-term) AW5133
    Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUFC2 Antibody (N-term)(Cat#AW5133). AW5133 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - NDUFC2 Antibody (N-term) AW5133
    Immunohistochemical analysis of paraffin-embedded R. kidney section using NDUFC2 Antibody (N-term)(Cat#AW5133). AW5133 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC, WB
Primary Accession O95298
Other Accession E9PQ53
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=14,11,10;M=14;Rat=14 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4718
Antigen Region 5-39
Other Names NADH dehydrogenase [ubiquinone] 1 subunit C2, Complex I-B145b, CI-B145b, Human lung cancer oncogene 1 protein, HLC-1, NADH-ubiquinone oxidoreductase subunit B145b, NDUFC2
Dilution WB~~1:1000
FC~~1:25
IF~~1:25
IHC-P~~1:25
Target/Specificity This NDUFC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-39 amino acids from the N-terminal region of human NDUFC2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFC2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFC2
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein; Matrix side
Research Areas

BACKGROUND

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

REFERENCES

Loeffen J.L.C.M.,et al.Biochem. Biophys. Res. Commun. 253:415-422(1998).
Dai F.Y.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Kim J.W.,et al.Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.

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