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ADRA1D Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ADRA1D Antibody (N-term) AW5131
    Western blot analysis of lysates from A549 cell line,rat brain tissue,LNCaP,PC-3 cell line (from left to right), using ADRA1D Antibody (N-term)(Cat. #AW5131). AW5131 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - ADRA1D Antibody (N-term) AW5131
    Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green, Cat#AW5131) compared to an isotype control of rabbit IgG(blue). AW5131 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - ADRA1D Antibody (N-term) AW5131
    Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term)(Cat#AW5131). AW5131 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ADRA1D Antibody (N-term) AW5131
    Immunohistochemical analysis of paraffin-embedded H. small intestine section using ADRA1D Antibody (N-term)(Cat#AW5131). AW5131 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ADRA1D Antibody (N-term) AW5131
    Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term)(Cat#AW5131). AW5131 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession P25100
Reactivity Human, Rat
Predicted Rabbit, Dog, Sheep, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=60;M=60;Rat=59 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 146
Antigen Region 1-30
Other Names Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A
Dilution WB~~1:1000
FC~~1:25
IHC-P~~1:25
Target/Specificity This ADRA1D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30amino acids from the N-terminal region of human ADRA1D.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADRA1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADRA1D
Synonyms ADRA1A
Function This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas

BACKGROUND

This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.

REFERENCES

Bruno J.F.,et al.Biochem. Biophys. Res. Commun. 179:1485-1490(1991).
Forray C.,et al.Mol. Pharmacol. 45:703-708(1994).
Schwinn D.A.,et al.J. Pharmacol. Exp. Ther. 272:134-142(1995).
Weinberg D.H.,et al.Biochem. Biophys. Res. Commun. 201:1296-1304(1994).
Esbenshade T.A.,et al.Mol. Pharmacol. 47:977-985(1995).

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