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HIST1H4A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HIST1H4A Antibody (C-term) AW5130
    Western blot analysis of lysates from A431,mouse NIH/3T3,L929,rat C6,Hela cell line (from left to right), using HIST1H4A Antibody (C-term)(Cat. #AW5130). AW5130 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - HIST1H4A Antibody (C-term) AW5130
    Flow cytometric analysis of MCF-7 cells using HIST1H4A Antibody (C-term)(green, Cat#AW5130) compared to an isotype control of rabbit IgG(blue). AW5130 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - HIST1H4A Antibody (C-term) AW5130
    Immunohistochemical analysis of paraffin-embedded H. skin section using HIST1H4A Antibody (C-term)(Cat#AW5130). AW5130 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HIST1H4A Antibody (C-term) AW5130
    Immunohistochemical analysis of paraffin-embedded R. skin section using HIST1H4A Antibody (C-term)(Cat#AW5130). AW5130 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HIST1H4A Antibody (C-term) AW5130
    Immunohistochemical analysis of paraffin-embedded M. skin section using HIST1H4A Antibody (C-term)(Cat#AW5130). AW5130 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HIST1H4A Antibody (C-term) AW5130
    Immunohistochemical analysis of paraffin-embedded H. esophagus section using HIST1H4A Antibody (C-term)(Cat#AW5130). AW5130 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HIST1H4A Antibody (C-term) AW5130
    Immunohistochemical analysis of paraffin-embedded M. esophagus section using HIST1H4A Antibody (C-term)(Cat#AW5130). AW5130 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession P62805
Other Accession P02309, P62799, P62804, P62802, P62806, Q4R362, P84040, P62801, P62784, P62803
Reactivity Human, Mouse, Rat
Predicted Monkey, Bovine, Chicken, Drosophila, C.Elegans, Xenopus
Host Rabbit
Clonality Polyclonal
Calculated MW H=11;M=11;Rat=11 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Antigen Region 71-103
Other Names Histone H4, HIST1H4A, H4/A, H4FA
Dilution WB~~1:1000
FC~~1:25
IHC-P~~1:25
Target/Specificity This HIST1H4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 71-103 amino acids from the C-terminal region of human HIST1H4A.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H4A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H4A
Synonyms H4/A, H4FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.

BACKGROUND

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

REFERENCES

Sierra F.,et al.Nucleic Acids Res. 11:7069-7086(1983).
Pauli U.,et al.Science 236:1308-1311(1987).
Albig W.,et al.Genomics 10:940-948(1991).
Drabent B.,et al.DNA Cell Biol. 14:591-597(1995).
Albig W.,et al.Gene 184:141-148(1997).

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