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HIST1H2AG Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HIST1H2AG Antibody (Center) AW5129
    Western blot analysis of lysates from mouse NIH/3T3,CEM,Hela,HepG2 cell line (from left to right), using HIST1H2AG Antibody (Center)(Cat. #AW5129). AW5129 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - HIST1H2AG Antibody (Center) AW5129
    Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (Center)(green, Cat#AW5129) compared to an isotype control of rabbit IgG(blue). AW5129 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - HIST1H2AG Antibody (Center) AW5129
    Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HIST1H2AG Antibody (Center) AW5129
    Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HIST1H2AG Antibody (Center) AW5129
    Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HIST1H2AG Antibody (Center) AW5129
    Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession P0C0S8
Other Accession P84051, P27661, P16104, Q7ZUY3, A9UMV8, Q8R1M2, Q4R3X5, Q9BTM1, P70082, Q3ZBX9, Q00728, P02263, Q4FZT6, Q8BFU2, Q7L7L0, P35062, P04912, Q64523, Q16777, A1A4R1, Q64522, Q8IUE6, P0CC09, Q6GSS7, Q6FI13, P04911, P06897, P02262, P22752, P0C0S9, Q8CGP7, Q99878
Reactivity Human, Mouse
Predicted Rat, Zebrafish, Monkey, Bovine, Chicken, Drosophila, Xenopus
Host Rabbit
Clonality Polyclonal
Calculated MW H=14;M=14 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 8329;8330;8332;8336;8969
Antigen Region 63-87
Other Names Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP
Dilution WB~~1:1000
FC~~1:25
IHC-P~~1:25
Target/Specificity This HIST1H2AG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-87 amino acids from the Central region of human HIST1H2AG.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H2AG Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H2AG
Synonyms H2AFP
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.

BACKGROUND

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

REFERENCES

Albig W.,et al.Hum. Genet. 101:284-294(1997).
Albig W.,et al.Biol. Chem. 380:7-18(1999).
Dobner T.,et al.DNA Seq. 1:409-413(1991).
Mannironi C.,et al.DNA Cell Biol. 13:161-170(1994).
Marzluff W.F.,et al.Genomics 80:487-498(2002).

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