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PSMD10 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PSMD10 Antibody (Center) AW5126
    Western blot analysis of lysates from MCF-7, PC-3, K562 cell line (from left to right), using PSMD10 Antibody (Center)(Cat. #AW5126). AW5126 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - PSMD10 Antibody (Center) AW5126
    Immunohistochemical analysis of paraffin-embedded H.testis section using PSMD10 Antibody (Center)(Cat#AP20569). AW5126 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PSMD10 Antibody (Center) AW5126
    Immunohistochemical analysis of paraffin-embedded H.lung adenocarcinoma section using PSMD10 Antibody (Center)(Cat#AW5126). AW5126 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PSMD10 Antibody (Center) AW5126
    Immunohistochemical analysis of paraffin-embedded M.testis section using PSMD10 Antibody (Center)(Cat#AW5126). AW5126 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - PSMD10 Antibody (Center) AW5126
    Fluorescent image of Hela cells stained with PSMD10 Antibody (Center)(Cat#AW5126). AW5126 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB
Primary Accession O75832
Other Accession Q9Z2X2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=24,16;M=25;Rat=25 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5716
Antigen Region 43-76
Other Names 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, Gankyrin, p28(GANK), PSMD10
Dilution WB~~ 1:1000
IHC-P~~1:25
IF~~1:25
Target/Specificity This PSMD10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 43-76 amino acids from the Central region of human PSMD10.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMD10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMD10
Function Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.
Cellular Location Cytoplasm. Nucleus
Tissue Location Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level).
Research Areas

BACKGROUND

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.

REFERENCES

Hori T.,et al.Gene 216:113-122(1998).
Higashitsuji H.,et al.Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
Wang H.,et al.Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

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