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HLA-B Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HLA-B Antibody (N-term) AW5124
    Western blot analysis of lysates from Jurkat,Ramos cell line,human spleen,rat spleen tissue lysate(from left to right), using HLA-B Antibody (N-term)(Cat. #AW5124). AW5124 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - HLA-B Antibody (N-term) AW5124
    Immunohistochemical analysis of paraffin-embedded H. tonsil section using HLA-B Antibody (N-term)(Cat#AW5124). AW5124 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HLA-B Antibody (N-term) AW5124
    Immunohistochemical analysis of paraffin-embedded H. spleen section using HLA-B Antibody (N-term)(Cat#AW5124). AW5124 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - HLA-B Antibody (N-term) AW5124
    Immunohistochemical analysis of paraffin-embedded R. spleen section using HLA-B Antibody (N-term)(Cat#AW5124). AW5124 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P30464
Other Accession P30488, P30486, P30483, P30479, Q04826, P30685, P30466
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=40 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Antigen Region 25-107
Other Names HLA class I histocompatibility antigen, B-15 alpha chain, MHC class I antigen B*15, HLA-B, HLAB
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-107 amino acids from the N-terminal region of human HLA-B.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-B
Synonyms HLAB
Function Involved in the presentation of foreign antigens to the immune system.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas

BACKGROUND

Involved in the presentation of foreign antigens to the immune system.

REFERENCES

Little A.-M.,et al.Tissue Antigens 38:186-190(1991).
Hildebrand W.H.,et al.Tissue Antigens 43:209-218(1994).
Lin L.,et al.Tissue Antigens 47:265-274(1996).
Madrigal J.A.,et al.J. Immunol. 149:3411-3415(1992).
Watkins D.I.,et al.Nature 357:329-333(1992).

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