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PRS4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PRS4 Antibody (C-term) AW5113
    Western blot analysis of lysates from A549,Hela,Jurkat,mouse NIH/3T3 cell line (from left to right), using PRS4 Antibody (C-term)(Cat. #AW5113). AW5113 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 1 - PRS4 Antibody (C-term) AW5113
    Western blot analysis of lysates from A549,Hela cell line,mouse lung,rat lung tissue (from left to right), using PRS4 Antibody (C-term)(Cat. #AW5113). AW5113 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 3 - PRS4 Antibody (C-term) AW5113
    Fluorescent image of Hela cells stained with PRS4 Antibody (C-term)(Cat#AW5113). AW5113 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - PRS4 Antibody (C-term) AW5113
    Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody (C-term)(Cat#AW5113). AW5113 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 3 - PRS4 Antibody (C-term) AW5113
    Fluorescent image of Hela cells stained with PRS4 Antibody (C-term)(Cat#AW5113). AW5113 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - PRS4 Antibody (C-term) AW5113
    Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody (C-term)(Cat#AW5113). AW5113 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P62191
Other Accession P62193, P62192
Reactivity Human, Mouse, Rat
Predicted Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=49;M=49;Rat=49 KDa
Isotype Rabbit Ig
Additional Information
Gene ID 5700
Antigen Region 408-436
Other Names 26S protease regulatory subunit 4;26S protease regulatory subunit 4; 26S protease regulatory subunit 4; 26S proteasome AAA-ATPase subunit RPT2; 26S protease regulatory subunit 4; Proteasome 26S subunit ATPase 1
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:25
Target/Specificity This PRS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human PRS4.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRS4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMC1
Function The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Cellular Location Cytoplasm. Nucleus.
Research Areas

BACKGROUND

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

REFERENCES

Dubiel W., et al. J. Biol. Chem. 267:22699-22702(1992).
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Matilla A., et al. Hum. Mol. Genet. 10:2821-2831(2001).
Brill L.M., et al. Anal. Chem. 76:2763-2772(2004).
Katiyar S., et al. Proc. Natl. Acad. Sci. U.S.A. 101:13774-13779(2004).

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