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ICT1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ICT1 Antibody (C-term) AW5108
    Western blot analysis of lysates from MDA-MB-453,NCI-H292,K562 cell line (from left to right), using ICT1 Antibody (C-term)(Cat. #AW5108). AW5108 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - ICT1 Antibody (C-term) AW5108
    Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using ICT1 Antibody (C-term)(Cat#AW5108). AW5108 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - ICT1 Antibody (C-term) AW5108
    Immunohistochemical analysis of paraffin-embedded H. skin section using ICT1 Antibody (C-term)(Cat#AW5108). AW5108 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession Q14197
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=24 KDa
Isotype Rabbit Ig
Additional Information
Gene ID 3396
Antigen Region 153-179
Other Names ICT1;DS1; Peptidyl-tRNA hydrolase ICT1, mitochondrial; Peptidyl-tRNA hydrolase ICT1, mitochondrial; Digestion substraction 1; Peptidyl-tRNA hydrolase ICT1, mitochondrial; Immature colon carcinoma transcript 1 protein
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This ICT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-179 amino acids from the C-terminal region of human ICT1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsICT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ICT1
Synonyms DS1
Function Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
Cellular Location Mitochondrion
Tissue Location Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells
Research Areas

BACKGROUND

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.

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