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MECR Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - MECR Antibody (Center) AW5107
    Western blot analysis of lysates from K562,Hela,HL-60,Jurkat cell line (from left to right), using MECR Antibody (Center)(Cat. #AW5107). AW5107 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9BV79
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB42835
Calculated MW H=40,32;M=40;Rat=40 KDa
Additional info
Gene ID 51102
Other Names MECR;NBRF1; Trans-2-enoyl-CoA reductase, mitochondrial; Trans-2-enoyl-CoA reductase, mitochondrial; Nuclear receptor-binding factor 1
Target/Specificity This MECR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-229 amino acids from the Central region of human MECR.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMECR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MECR
Synonyms NBRF1
Function Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2- decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis.
Cellular Location Isoform 1: Mitochondrion
Tissue Location Highly expressed in skeletal and heart muscle. Expressed at lower level in placenta, liver, kidney and pancreas Weakly or not expressed in lung.
Research Areas

BACKGROUND

Catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. May have a role in the mitochondrial synthesis of fatty acids.

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