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DMRTA2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DMRTA2 Antibody (C-term) AW5104
    Western blot analysis of lysates from K562 cell line,zebra fish brain,mouse brain,mouse testis tissue lysate,PC-3 cell line (from left to right), using DMRTA2 Antibody (C-term)(Cat. #AW5104). AW5104 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 3 - DMRTA2 Antibody (C-term) AW5104
    Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)(Cat#AW5104).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF
Primary Accession Q96SC8
Other Accession A2A9A2, A6QQ94, NP_115486.1
Reactivity Human, Mouse, Zebrafish
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB41920
Calculated MW H=53;M=53;Z=47 KDa
Additional info
Gene ID 63950
Other Names DMRTA2; DMRT5; Doublesex- and mab-3-related transcription factor A2; Doublesex- and mab-3-related transcription factor 5
Target/Specificity This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDMRTA2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DMRTA2
Synonyms DMRT5
Function May be involved in sexual development.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00070}
Tissue Location Expressed in testis.
Research Areas

BACKGROUND

DMRTA2 may be involved in sexual development.

REFERENCES

Ottolenghi, C., et al. Genomics 79(3):333-343(2002)

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