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TAF7 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - TAF7 Antibody (C-term) AW5093
    Western blot analysis of lysates from A549,Hela cell line (from left to right), using TAF7 Antibody (C-term)(Cat. #AW5093). AW5093 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q15545
Other Accession Q9R1C0, Q4R5A5, Q6R1L1, Q2HJG8, NP_005633.2
Reactivity Human
Predicted Mouse, Hamster, Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=40;M=39 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 6879
Antigen Region 308-337
Other Names TAF7; TAF2F; TAFII55; Transcription initiation factor TFIID subunit 7; RNA polymerase II TBP-associated factor subunit F; Transcription initiation factor TFIID 55 kDa subunit
Dilution WB~~1:1000
Target/Specificity This TAF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-337 amino acids from the C-terminal region of human TAF7.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF7
Synonyms TAF2F, TAFII55
Function Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
Cellular Location Nucleus.
Tissue Location Ubiquitous.

BACKGROUND

The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II.

REFERENCES

Gegonne, A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(14):5367-5372(2008)
Hartman, W.R., et al. Arch. Biochem. Biophys. 459(2):223-232(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Couture, J.F., et al. Nat. Struct. Mol. Biol. 13(2):140-146(2006)

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