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HAND1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HAND1 Antibody (Center) AW5092
    Western blot analysis of lysates from HT-29,SK-BR-3,SW480 cell line (from left to right), using HAND1 Antibody (Center)(Cat. #AW5092). AW5092 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O96004
Other Accession P57100, Q0VCE2, NP_004812.1
Reactivity Human
Predicted Rat, Rabbit, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB34361
Calculated MW H=24;Rat=24 KDa
Additional info
Gene ID 9421
Other Names HAND1; BHLHA27; EHAND; Heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1
Target/Specificity This HAND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human HAND1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHAND1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAND1
Synonyms BHLHA27, EHAND
Function Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box) (By similarity).
Cellular Location Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity).
Tissue Location Heart.
Research Areas

BACKGROUND

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.

REFERENCES

Reamon-Buettner, S.M., et al. Hum. Mol. Genet. 18(19):3567-3578(2009) Martinez Hoyos, J., et al. Oncogene 28(6):876-885(2009) Reamon-Buettner, S.M., et al. Hum. Mol. Genet. 17(10):1397-1405(2008) Morin, S., et al. J. Biol. Chem. 280(37):32272-32278(2005) Hill, A.A., et al. Mol. Cell. Biol. 24(22):9835-9847(2004)

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