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TSN Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - TSN Antibody (Center) AW5088
    Western blot analysis of lysates from K562,Jurkat,Hela cell line (from left to right), using TSN Antibody(Center)(Cat. #AW5088). AW5088 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - TSN Antibody (Center) AW5088
    TSN Antibody (Center) (Cat. #AW5088)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - TSN Antibody (Center) AW5088
    Fluorescent confocal image of Hela cell stained with TSN Antibody(Center)(Cat#AW5088).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P
Primary Accession Q15631
Other Accession Q62348, P97891, Q08DM8, NP_004613.1
Reactivity Human
Predicted Mouse, Hamster, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB31284
Calculated MW H=26;M=26 KDa
Additional info
Gene ID 7247
Other Names TSN; Translin; Component 3 of promoter of RISC
Target/Specificity This TSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human TSN.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSN
Function DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.
Cellular Location Cytoplasm. Nucleus

BACKGROUND

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms.

REFERENCES

Chiaruttini, C., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16481-16486(2009)
Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008) :
Sengupta, K., et al. Biochemistry 45(3):861-870(2006)
Kaluzhny, D., et al. J. Biomol. Struct. Dyn. 23(3):257-265(2005)
Gupta, G.D., et al. FEBS Lett. 579(14):3141-3146(2005)

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