|Application ||IF, WB|
|Other Accession||Q7SZ22, Q5XIF4, Q9Z172, Q6DI05, Q17QV3, P61959, P61958, P61957, Q2PFW2, P61956, Q6DHL4, Q6LDZ8, Q5ZJM9, P61955, Q6NV25, Q6GPW2, Q7ZTK7|
|Predicted||Rat, Zebrafish, Hamster, Monkey, Bovine, Chicken, Xenopus|
|Calculated MW||H=12;M=12;Rat=12 KDa|
|Other Names||SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B|
|Target/Specificity||This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-81 amino acids from the C-terminal region of human SUMO2/3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SUMO2/3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus, PML body|
|Tissue Location||Expressed predominantly in liver.|
SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Lapenta, V., et al., Genomics 40(2):362-366 (1997).