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RPS6 Antibody (N-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - RPS6 Antibody (N-term) AW5080
    Western blot analysis of lysates from mouse NIH/3T3,Hela cell line,rat brain tissue lysate (from left to right), using RPS6 Antibody (N-term)(Cat. #AW5080). AW5080 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - RPS6 Antibody (N-term) AW5080
    Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green, Cat#AW5080) compared to an isotype control of mouse IgG1(blue). AW5080 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - RPS6 Antibody (N-term) AW5080
    Fluorescent image of Hela cells stained with RPS6 Antibody (N-term)(Cat#AW5080). AW5080 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - RPS6 Antibody (N-term) AW5080
    Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term)(Cat#AW5080). AW5080 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - RPS6 Antibody (N-term) AW5080
    Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term)(Cat#AW5080). AW5080 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - RPS6 Antibody (N-term) AW5080
    Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term)(Cat#AW5080). AW5080 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC, WB
Primary Accession P62753
Other Accession P62755, P62754, Q4R4K6
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Mouse
Clonality Monoclonal
Calculated MW H=29;M=29;Rat=29 KDa
Isotype IgG1
Antigen Source HUMAN
Additional Information
Gene ID 6194
Antigen Region 1-239
Other Names 40S ribosomal protein S6, Phosphoprotein NP33, RPS6
Dilution WB~~1:1000
FC~~1:25
IF~~1:25
IHC-P~~1:25
Target/Specificity This RPS6 antibody is generated from a mouse immunized with RPS6 recombinant protein.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS6
Function May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Research Areas

BACKGROUND

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

REFERENCES

Lott J.B.,et al.Gene 65:31-39(1988).
Heinze H.,et al.J. Biol. Chem. 263:4139-4144(1988).
Antoine M.,et al.Hum. Mol. Genet. 1:565-570(1992).
Pata I.,et al.Gene 121:387-392(1992).
Shichijo S.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.

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