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PPARA Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - PPARA Antibody AW5078
    Western blot analysis of lysates from Hela,Jurkat,mouse NIH/3T3 cell line (from left to right), using PPARA Antibody(Cat. #AW5078). AW5078 was diluted at 1:500 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - PPARA Antibody AW5078
    Flow cytometric analysis of Hela cells using PPARA Antibody(green, Cat#AW5078) compared to an isotype control of mouse IgG1(blue). AW5078 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - PPARA Antibody AW5078
    Fluorescent image of Hela cells stained with PPARA Antibody(Cat#AW5078). AW5078 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - PPARA Antibody AW5078
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using PPARA Antibody(Cat#AW5078). AW5078 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PPARA Antibody AW5078
    Immunohistochemical analysis of paraffin-embedded H. kidney section using PPARA Antibody(Cat#AW5078). AW5078 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC, WB
Primary Accession Q07869
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Calculated MW H=52;M=52 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 5465
Other Names Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, PPARA, NR1C1, PPAR
Dilution WB~~1:500
FC~~1:25
IF~~1:25
IHC-P~~1:25
Target/Specificity This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPARA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPARA
Synonyms NR1C1, PPAR
Function Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Cellular Location Nucleus.
Tissue Location Skeletal muscle, liver, heart and kidney.
Research Areas

BACKGROUND

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

REFERENCES

Sher T.,et al.Biochemistry 32:5598-5604(1993).
Mukherjee R.,et al.J. Steroid Biochem. Mol. Biol. 51:157-166(1994).
Tugwood J.D.,et al.Ann. N. Y. Acad. Sci. 804:252-265(1996).
Kobayashi T.,et al.FEBS Lett. 582:2737-2744(2008).
Cho M.-C.,et al.Immunopharmacol. Immunotoxicol. 31:459-467(2009).

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