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USP5 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - USP5 Antibody AW5077
    Western blot analysis of lysates from mouse brain tissue,PC-3 cell line,rat liver tissue,U-251 MG cell line,rat brain tissue (from left to right), using USP5 Antibody(Cat. #AW5077). AW5077 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P45974
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=96;M=96;Rat=96 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 8078
Antigen Region 1-160
Other Names Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5, USP5, ISOT
Dilution WB~~1:1000
Target/Specificity This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP5
Synonyms ISOT
Function Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Research Areas

BACKGROUND

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

REFERENCES

Falquet L.,et al.FEBS Lett. 376:233-237(1995).
Ansari-Lari M.A.,et al.Genome Res. 6:314-326(1996).
Ansari-Lari M.A.,et al.Genome Res. 7:268-280(1997).
Tashayev V.L.,et al.Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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