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RAD51 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - RAD51 Antibody AW5075
    Western blot analysis of lysates from Jurkat,mouse NIH/3T3,rat C6,rat PC-12,Hela cell line (from left to right), using RAD51 Antibody(Cat. #AW5075). AW5075 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q06609
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=37,26,31 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 5888
Other Names DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A, RAD51, RAD51A, RECA
Dilution WB~~1:1000
Target/Specificity This RAD51 antibody is generated from a mouse immunized with a recombination protein from human.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD51 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD51
Synonyms RAD51A, RECA
Function Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Cellular Location Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment Accumulated at sites of DNA damage in a SPIDR-dependent manner
Tissue Location Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary Weakly expressed in breast

BACKGROUND

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

REFERENCES

Shinohara A.,et al.Nat. Genet. 4:239-243(1993).
Yoshimura Y.,et al.Nucleic Acids Res. 21:1665-1665(1993).
Schmutte C.,et al.Cancer Res. 59:4564-4569(1999).
Wang W.W.,et al.Cancer Epidemiol. Biomarkers Prev. 10:955-960(2001).
Park J.Y.,et al.Nucleic Acids Res. 36:3226-3234(2008).

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