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PAH Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - PAH Antibody (Center) AW5074
    Western blot analysis of lysates from mouse liver,rat liver tissue,HepG2 cell line (from left to right), using PAH Antibody (Center)(Cat. #AW5074). AW5074 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - PAH Antibody (Center) AW5074
    Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center)(Cat#AW5074). AW5074 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PAH Antibody (Center) AW5074
    Immunohistochemical analysis of paraffin-embedded H. kidney section using PAH Antibody (Center)(Cat#AW5074). AW5074 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PAH Antibody (Center) AW5074
    Immunohistochemical analysis of paraffin-embedded M. kidney section using PAH Antibody (Center)(Cat#AW5074). AW5074 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P00439
Other Accession P04176, P16331, Q2KIH7
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Mouse
Clonality Monoclonal
Calculated MW H=52;M=52;Rat=52 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 5053
Antigen Region 127-161
Other Names Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase, PAH
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAH
Research Areas

REFERENCES

Kwok S.C.M.,et al.Biochemistry 24:556-561(1985).
Scriver C.R.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Cotton R.G.,et al.Biochem. J. 255:193-196(1988).
Miranda F.F.,et al.J. Biol. Chem. 277:40937-40943(2002).
Siltberg-Liberles J.,et al.Gene 427:86-92(2008).

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