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GATA3 Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - GATA3 Antibody (Center) AW5071
    Western blot analysis of lysates from CEM, SH-SY5Y cell line (from left to right), using GATA3 Antibody (Center)(Cat. #AW5071). AW5071 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - GATA3 Antibody (Center) AW5071
    Flow cytometric analysis of MCF-7 cells using GATA3 Antibody (Center)(green, Cat#AW5071) compared to an isotype control of mouse IgG2b(blue). AW5071 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - GATA3 Antibody (Center) AW5071
    Fluorescent image of MCF-7 cells stained with GATA3 Antibody (Center)(Cat#AW5071). AW5071 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC
Primary Accession P23771
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b,κ
Clone Names 1259CT707.136.102
Calculated MW H=48 KDa
Additional info
Gene ID 2625
Other Names Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3
Target/Specificity This GATA3 antibody is generated from a mouse immunized with a recombinant protein from human GATA3.
Dilution WB~~ 1:1000
FC~~1:25
IF~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGATA3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GATA3
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Cellular Location Nucleus.
Tissue Location T-cells and endothelial cells.
Research Areas

BACKGROUND

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.

REFERENCES

Joulin V.,et al.EMBO J. 10:1809-1816(1991).
Ho I.-C.,et al.EMBO J. 10:1187-1192(1991).
Ko L.J.,et al.Mol. Cell. Biol. 11:2778-2784(1991).
Marine J.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:7284-7288(1991).
Deloukas P.,et al.Nature 429:375-381(2004).

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