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PHB Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - PHB Antibody AW5065
    Western blot analysis of lysates from HepG2 cell line,rat liver tissue,MCF-7,mouse NIH/3T3 cell line (from left to right), using PHB Antibody(Cat. #AW5065). AW5065 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - PHB Antibody AW5065
    Immunohistochemical analysis of paraffin-embedded H. stomach section using PHB Antibody(Cat#AW5065). AW5065 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P35232
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=30;M=30;Rat=30 KDa
Isotype IgG2b,k
Antigen Source HUMAN
Additional Information
Gene ID 5245
Antigen Region 1-205
Other Names Prohibitin, PHB
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This antibody is generated from a mice immunized with a recombinant protein between 1-205 amino acids from human.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHB
Function Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Cellular Location Mitochondrion inner membrane.
Tissue Location Widely expressed in different tissues.
Research Areas

BACKGROUND

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

REFERENCES

Sato T.,et al.Cancer Res. 52:1643-1646(1992).
Sato T.,et al.Genomics 17:762-764(1993).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Bienvenut W.V.,et al.Submitted (MAR-2005) to UniProtKB.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.

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