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Creatine Kinase BB Antibody (Center)

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - Creatine Kinase BB Antibody  (Center) AW5061
    Western blot analysis of lysates from MDA-MB-453,Y79 cell line,mouse stomach,mouse brain tissue lysate(from left to right), using Creatine Kinase BB (CKB) Antibody (Center)(Cat. #AW5061). AW5061 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P12277
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 893CT29.1.1
Calculated MW H=43;M=43 KDa
Additional info
Gene ID 1152
Other Names CKB;CKBB; Creatine kinase B-type; Creatine kinase B-type; B-CK; Creatine kinase B-type; Creatine kinase B chain
Target/Specificity Purified His-tagged CKB protein was used to produced this monoclonal antibody.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCreatine Kinase BB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CKB
Synonyms CKBB
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

REFERENCES

Villarreal-Levy G., et al. Biochem. Biophys. Res. Commun. 144:1116-1127(1987).
Mariman E.C.M., et al. Genomics 1:126-137(1987).
Kaye F.J., et al. J. Clin. Invest. 79:1412-1420(1987).
Mariman E.C.M., et al. Nucleic Acids Res. 17:6385-6385(1989).
Ota T., et al. Nat. Genet. 36:40-45(2004).

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