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CREB1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - CREB1 Antibody AW5058
    Western blot analysis of lysates from A549,mouse Neuro-2a,NIH/3T3,rat C6 cell line (from left to right), using CREB1 Antibody(Cat. #AW5058). AW5058 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P16220
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 1335CT115.203.189
Calculated MW H=37,35;M=37,35;Rat=37 KDa
Additional info
Gene ID 1385
Other Names Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Target/Specificity This CREB1 antibody is generated from a mouse immunized with a recombinant protein from human CREB1.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCREB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CREB1
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083}
Research Areas

BACKGROUND

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

REFERENCES

Berkowitz L.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5258-5262(1990).
Yoshimura T.,et al.EMBO J. 9:2537-2542(1990).
Waeber G.,et al.Trans. Assoc. Am. Physicians 103:28-37(1990).
Hoeffler J.P.,et al.Science 242:1430-1433(1988).
Short M.L.,et al.Nucleic Acids Res. 19:4290-4290(1991).

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