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P100 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - P100 Antibody AW5052
    Western blot analysis of lysates from HT-29,Jurkat,MCF-7 cell line (from left to right), using P100 Antibody(Cat. #AW5052). AW5052 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9ULW0
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=86 KDa
Isotype Mouse IgG1
Antigen Source HUMAN
Additional Information
Gene ID 22974
Antigen Region Multiple
Other Names TPX2; C20orf1; C20orf2; DIL2; HCA519; Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; Hepatocellular carcinoma-associated antigen 519; Protein fls353; Restricted expression proliferation-associated protein 100
Dilution WB~~1:1000
Target/Specificity This monoclonal antibody is generated from mice immunized with a collection of antigenic peptides from different regions of P100.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsP100 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPX2
Synonyms C20orf1, C20orf2, DIL2, HCA519
Function Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules
Tissue Location Expressed in lung carcinoma cell lines but not in normal lung tissues
Research Areas

REFERENCES

Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.
A cancer-associated aurora A mutant is mislocalized and misregulated due to loss of interaction with TPX2. Bibby RA, et al. J Biol Chem, 2009 Nov 27. PMID 19801554.
Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Hosgood HD 3rd, et al. Occup Environ Med, 2009 Dec. PMID 19773279.
Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas. Shigeishi H, et al. Int J Oncol, 2009 Jun. PMID 19424574.
Dynamic release of nuclear RanGTP triggers TPX2-dependent microtubule assembly during the apoptotic execution phase. Moss DK, et al. J Cell Sci, 2009 Mar 1. PMID 19208764.

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