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TBB5 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - TBB5 Antibody AW5051
    Western blot analysis of lysates from mouse brain tissue, Hela,Y79,CEM,rat PC-12 cell line (from left to right), using TBB5 Antibody(Cat. #AW5051). AW5051 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - TBB5 Antibody AW5051
    Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - TBB5 Antibody AW5051
    Immunofluorescent analysis of A549 cells, using TBB5 Antibody (Cat. #AW5051). AW5051 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
  • 14 - TBB5 Antibody AW5051
    Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody(Cat#AW5051). AW5051 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P07437
Other Accession P09244, Q91575, P69897, Q767L7, P99024, P69893, Q2KJD0
Reactivity Human, Mouse, Rat
Predicted Hamster, Bovine, Chicken, Xenopus
Host Mouse
Clonality Monoclonal
Calculated MW H=50;M=50;Rat=50 KDa
Isotype IgG2b,κ
Antigen Source HUMAN
Additional Information
Gene ID 203068
Antigen Region 1-219
Other Names TUBB; TUBB5; Tubulin beta chain; Tubulin beta-5 chain
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Target/Specificity TBB5 recombinant protein is used to produce this monoclonal antibody.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBB5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB
Synonyms TUBB5
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research Areas

REFERENCES

An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014. High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions. Barcellos LF, et al. PLoS Genet, 2009 Oct. PMID 19851445. Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation. Martins-de-Souza D, et al. BMC Psychiatry, 2009 Apr 30. PMID 19405953. Identification of interaction partners for individual SH3 domains of Fas ligand associated members of the PCH protein family in T lymphocytes. Linkermann A, et al. Biochim Biophys Acta, 2009 Feb. PMID 19041431. Alterations in oligodendrocyte proteins, calcium homeostasis and new potential markers in schizophrenia anterior temporal lobe are revealed by shotgun proteome analysis. Martins-de-Souza D, et al. J Neural Transm, 2009 Mar. PMID 19034380.

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Anonymous
2016-08-24 09:20:44
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Species tested
Human,Mouse
Application tested
WB
Organization tested
Hela,PC12,U87,HT-29,MCF-7,Brain(M),Spleen(M),Thymus(M),Spleen(R)
Barcode encoding
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose