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EEF1B2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - EEF1B2 Antibody (Center) AW5048
    Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using EEF1B2 Antibody (Center)(Cat. #AW5048). AW5048 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - EEF1B2 Antibody (Center) AW5048
    Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody (Center)(Cat#AW5048). AW5048 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - EEF1B2 Antibody (Center) AW5048
    Fluorescent image of Hela cells stained with EEF1B2 Antibody (Center)(Cat#AW5048). AW5048 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 4 - EEF1B2 Antibody (Center) AW5048
    Flow cytometric analysis of Hela cells using EEF1B2 Antibody (Center)(green, Cat#AW5048) compared to an isotype control of rabbit IgG(blue). AW5048 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IF, IHC-P
Primary Accession P24534
Other Accession Q9YGQ1
Reactivity Human
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB48012
Calculated MW H=25 KDa
Additional info
Gene ID 1933
Other Names Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B
Target/Specificity This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2.
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1B2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1B2
Synonyms EEF1B, EF1B
Function EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

BACKGROUND

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

REFERENCES

Sanders J.,et al.Nucleic Acids Res. 19:4551-4551(1991).
von der Kammer H.,et al.Biochem. Biophys. Res. Commun. 177:312-317(1991).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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