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AK4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - AK4 Antibody (Center) AW5045
    Western blot analysis of lysates from A431, HepG2 cell line, mouse kidney tissue, MCF-7, Raji, Hela cell line (from left to right), using AK4 Antibody (Center)(Cat. #AW5045). AW5045 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - AK4 Antibody (Center) AW5045
    Immunohistochemical analysis of paraffin-embedded H.heart section using AK4 Antibody (Center)(Cat#AW5045). AW5045 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - AK4 Antibody (Center) AW5045
    Immunohistochemical analysis of paraffin-embedded M.kidney section using AK4 Antibody (Center)(Cat#AW5045). AW5045 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - AK4 Antibody (Center) AW5045
    Immunohistochemical analysis of paraffin-embedded H.kidney section using AK4 Antibody (Center)(Cat#AW5045). AW5045 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - AK4 Antibody (Center) AW5045
    Fluorescent image of HepG2 cells stained with AK4 Antibody (Center)(Cat#AW5045). AW5045 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB
Primary Accession P27144
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=25;M=25;Rat=25 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 100507855;205
Antigen Region 119-153
Other Names Adenylate kinase 4, mitochondrial, AK 4, Adenylate kinase 3-like, GTP:AMP phosphotransferase AK4, AK4, AK3, AK3L1
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Target/Specificity This AK4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-153 amino acids from the Central region of human AK4.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAK4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AK4 {ECO:0000255|HAMAP-Rule:MF_03170}
Function Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.
Cellular Location Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03170, ECO:0000269|PubMed:11485571, ECO:0000269|PubMed:19766732}
Tissue Location Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain
Research Areas

BACKGROUND

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.

REFERENCES

Xu G.,et al.Genomics 13:537-542(1992).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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