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CYC1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CYC1 Antibody (Center) AW5042
    Western blot analysis of lysates from A431, Hela, HepG2 cell line, mouse brain tissue and MCF-7, U-251 MG cell line (from left to right), using CYC1 Antibody (Center)(Cat. #AW5042). AW5042 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - CYC1 Antibody (Center) AW5042
    Immunohistochemical analysis of paraffin-embedded M. stomach section using CYC1 Antibody (Center)(Cat#AW5042). AW5042 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - CYC1 Antibody (Center) AW5042
    Immunohistochemical analysis of paraffin-embedded H. stomach section using CYC1 Antibody (Center)(C-term)(Cat#AW5042). AW5042 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - CYC1 Antibody (Center) AW5042
    Fluorescent image of Hela cells stained with CYC1 Antibody (Center)(Cat#AW5042). AW5042 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - CYC1 Antibody (Center) AW5042
    Immunohistochemical analysis of paraffin-embedded R. stomach section using CYC1 Antibody (Center)(Cat#AW5042). AW5042 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF
Primary Accession P08574
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB48502
Calculated MW H=35;M=35,29 KDa
Additional info
Gene ID 1537
Other Names Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
Target/Specificity This CYC1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 142-176 amino acids from the Central region of human CYC1.
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYC1
Function This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side
Research Areas

BACKGROUND

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

REFERENCES

Nishikimi M.,et al.Nucleic Acids Res. 16:3577-3577(1988).
Suzuki H.,et al.J. Biol. Chem. 264:1368-1374(1989).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Nusbaum C.,et al.Nature 439:331-335(2006).

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