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Mouse Mapk3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Mouse Mapk3 Antibody (C-term) AW5034
    Western blot analysis of lysates from mouse NIH/3T3,BA/F3,mouse C2C12,rat C6 cell line (from left to right),using Mouse Mapk3 Antibody (C-term)(Cat. #AW5034). AW5034 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - Mouse Mapk3 Antibody (C-term) AW5034
    Mouse Mapk3 Antibody (C-term) (AW5034)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - Mouse Mapk3 Antibody (C-term) AW5034
    Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green, Cat#AW5034) compared to an isotype control of rabbit IgG(blue). AW5034 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession Q63844
Other Accession NP_036082.1
Reactivity Mouse, Rat
Predicted Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=43;M=43;Rat=43 KDa
Isotype Rabbit Ig
Antigen Source MOUSE
Additional Information
Gene ID 26417
Antigen Region 353-380
Other Names Mapk3; Erk1; Prkm3; Mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; MNK1; Microtubule-associated protein 2 kinase; p44-ERK1
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:25
Target/Specificity This Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of mouse Mapk3.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Mapk3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mapk3
Synonyms Erk1, Prkm3
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Cellular Location Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization (By similarity). PEA15- binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm.
Research Areas

BACKGROUND

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) and heat shock factor protein 4 (HSF4) (By similarity). Phosphorylates SPZ1.

REFERENCES

Chen, W., et al. Biochem. Biophys. Res. Commun. 401(3):339-343(2010)
Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
Alter, B.J., et al. J. Neurosci. 30(34):11537-11547(2010)
Spruce, T., et al. Dev. Cell 19(2):207-219(2010)
Bremer, J., et al. PLoS ONE 5 (8), E12450 (2010) :

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