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VAPB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - VAPB Antibody (Center) AW5033
    Western blot analysis of lysates from HepG2, Hela, SH-SY5Y, mouse NIH/3T3 cell line and rat heart tissue lysate(from left to right),using VAPB Antibody (Center)(Cat. #AW5033). AW5033 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O95292
Other Accession A5GFS8, A2VDZ9
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality polyclonal
Calculated MW H=27;M=27;Rat=27 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 9217
Antigen Region 126-139
Other Names Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C, VAPB
Dilution WB~~1:1000
Target/Specificity This VAPB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 126-139 amino acids from the Central region of human VAPB.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVAPB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VAPB
Function Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.
Cellular Location Endoplasmic reticulum membrane; Single-pass type IV membrane protein Note=Present in mitochondria-associated membranes that are endoplasmic reticulum membrane regions closely apposed to the outer mitochondrial membrane.
Tissue Location Ubiquitous. Isoform 1 predominates.
Research Areas

BACKGROUND

Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.

REFERENCES

Nishimura Y.,et al.Biochem. Biophys. Res. Commun. 254:21-26(1999).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Deloukas P.,et al.Nature 414:865-871(2001).
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).

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