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NDUFB4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NDUFB4 Antibody (N-term) AW5032
    Western blot analysis of lysates from human heart tissue, HepG2, PC-3, Ramos cell line (from left to right), using NDUFB4 Antibody (N-term)(Cat. #AW5032). AW5032 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - NDUFB4 Antibody (N-term) AW5032
    Flow cytometric analysis of Hela cells using NDUFB4 Antibody (N-term)(green, Cat#AW5032) compared to an isotype control of rabbit IgG(blue). AW5032 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - NDUFB4 Antibody (N-term) AW5032
    Fluorescent image of Hela cells stained with NDUFB4 Antibody (N-term)(Cat#AW5032). AW5032 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - NDUFB4 Antibody (N-term) AW5032
    Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term)(Cat#AW5032). AW5032 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC
Primary Accession O95168
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB49182
Calculated MW H=15,14 KDa
Additional info
Gene ID 4710
Other Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, CI-B15, NADH-ubiquinone oxidoreductase B15 subunit, NDUFB4
Target/Specificity This NDUFB4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human NDUFB4.
Dilution WB~~1:1000
FC~~1:25
IF~~1:25
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFB4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFB4
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein; Matrix side
Research Areas

BACKGROUND

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

REFERENCES

Loeffen J.L.C.M.,et al.Biochem. Biophys. Res. Commun. 253:415-422(1998).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Murray J.,et al.J. Biol. Chem. 278:13619-13622(2003).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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