|Application ||IHC-P, WB|
|Calculated MW||H=56;M=56;Rat=56 KDa|
|Other Names||PACSIN2; Protein kinase C and casein kinase substrate in neurons protein 2|
|Target/Specificity||This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the C-terminal region of human PACSIN2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PACSIN2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle- mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome Recycling endosome membrane. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Membrane, caveola. Note=Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1|
|Tissue Location||Widely expressed.|
PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Wiemann, S., et al., Genome Res. 11(3):422-435 (2001). Ritter, B., et al., FEBS Lett. 454(3):356-362 (1999). Dunham, I., et al., Nature 402(6761):489-495 (1999).