注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   SMAD1 Antibody (Center)   

SMAD1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - SMAD1 Antibody (Center) AW5021
    Western blot analysis of lysates from Hela,HepG2,HT-1080, mouse C2C12 cell line and rat liver tissue lysate (from left to right), using SMAD1 Antibody (Center)(Cat. #AW5021). AW5021 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - SMAD1 Antibody (Center) AW5021
    Immunohistochemical analysis of paraffin-embedded H. heart section using SMAD1 Antibody (Center)(Cat#AW5021). AW5021 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - SMAD1 Antibody (Center) AW5021
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SMAD1 Antibody (Center)(Cat#AW5021). AW5021 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - SMAD1 Antibody (Center) AW5021
    Fluorescent image of MCF-7 cells stained with SMAD1 Antibody (Center)(Cat#AW5021). AW5021 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 产品详情
  • 实验流程
  • 背景知识
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB
Primary Accession Q15797
Other Accession P97588, P70340, Q1JQA2
Reactivity Human, Rat
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality polyclonal
Calculated MW H=52;M=52;Rat=53 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4086
Antigen Region 163-196
Other Names Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD 1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1, SMAD1, BSP1, MADH1, MADR1
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Target/Specificity This SMAD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMAD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD1
Synonyms BSP1, MADH1, MADR1
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane
Tissue Location Ubiquitous. Highest expression seen in the heart and skeletal muscle
Research Areas

BACKGROUND

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

REFERENCES

Riggins G.J.,et al.Nat. Genet. 13:347-349(1996).
Liu F.,et al.Nature 381:620-623(1996).
Hoodless P.A.,et al.Cell 85:489-500(1996).
Lechleider R.J.,et al.J. Biol. Chem. 271:17617-17620(1996).
Zhang Y.,et al.Nature 383:168-172(1996).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69369083 tech.abgent@wuxiapptec.com.


我要评价