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CTSD Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CTSD Antibody AW5009
    Western blot analysis of lysates from HepG2, MCF-7, A431 cell line (from left to right), using CTSD Antibody(Cat. #AW5009). AW5009 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.
  • 14 - CTSD Antibody AW5009
    Immunohistochemical analysis of paraffin-embedded H.liver section using CTSD(Cat#AW5009). AW5009 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - CTSD Antibody AW5009
    Immunohistochemical analysis of paraffin-embedded H.breast carcinoma section using CTSD(Cat#AW5009). AW5009 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - CTSD Antibody AW5009
    Fluorescent image of HepG2 cells stained with CTSD(Cat#AW5009). AW5009 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P
Primary Accession P07339
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Clone Names RB44366
Calculated MW 44552 Da
Additional info
Gene ID 1509
Other Names Cathepsin D, Cathepsin D light chain, Cathepsin D heavy chain, CTSD, CPSD
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTSD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTSD
Synonyms CPSD
Function Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Cellular Location Lysosome. Melanosome. Secreted, extracellular space. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
Tissue Location Expressed in the aorta extrcellular space (at protein level).
Research Areas

BACKGROUND

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

REFERENCES

Faust P.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:4910-4914(1985).
Westley B.R.,et al.Nucleic Acids Res. 15:3773-3786(1987).
Redecker B.,et al.DNA Cell Biol. 10:423-431(1991).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

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