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ATP5I Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ATP5I Antibody (C-term) AW5003
    Western blot analysis of lysates from mouse liver, heart tissue and A431 cell line (from left to right), using ATP5I Antibody (C-term)(Cat. #AW5003). AW5003 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.
  • 14 - ATP5I Antibody (C-term) AW5003
    Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ATP5I Antibody (C-term) AW5003
    Immunohistochemical analysis of paraffin-embedded M. heart section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ATP5I Antibody (C-term) AW5003
    Immunohistochemical analysis of paraffin-embedded H. kidney section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - ATP5I Antibody (C-term) AW5003
    Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 3 - ATP5I Antibody (C-term) AW5003
    Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB
Primary Accession P56385
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW H=8;M=8 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 521
Antigen Region 55-89
Other Names ATP synthase subunit e, mitochondrial, ATPase subunit e, ATP5I, ATP5K
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Target/Specificity This ATP5I antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 55-89 amino acids from the C-terminal region of human ATP5I.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP5I Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP5I
Synonyms ATP5K
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Cellular Location Mitochondrion. Mitochondrion inner membrane.
Research Areas

BACKGROUND

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

REFERENCES

Fujiwara T.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Xu G.,et al.Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).

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