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SMAD3-S208 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SMAD3-S208 Antibody AP9995a
    SMAD3 Antibody (S208) (Cat. #AP9995a) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).
  • 14 - SMAD3-S208 Antibody AP9995a
    SMAD3-S208 Antibody (cat. #AP9995a) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - SMAD3-S208 Antibody AP9995a
    Confocal immunofluorescent analysis of SMAD3-S208 Antibody(Cat#AP9995a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
  • 4 - SMAD3-S208 Antibody AP9995a
    SMAD3-S208 Antibody (Cat. #AP9995a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC, WB, E
Primary Accession P84022
Other Accession P84025, P84024, Q8BUN5, P84023
Reactivity Human, Mouse
Predicted Mouse, Rat, Pig, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4088
Other Names Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3
Target/Specificity This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMAD3-S208 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD3
Synonyms MADH3
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236)
Research Areas
The let-7g microRNA promotes follicular granulosa cell apoptosis by targeting transforming growth factor-β type 1 receptor.
Author : Zhou J1, Liu J1, Pan Z1, Du X1, Li X1, Ma B1, Yao W1, Li Q2, Liu H3.
Mol Cell Endocrinol. 2015 Jul 5;409:103-12. doi: 10.1016/j.mce.2015.03.012. Epub 2015 Mar 26.
25817543
Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks.
Author : Lessard SJ1, Rivas DA, Alves-Wagner AB, Hirshman MF, Gallagher IJ, Constantin-Teodosiu D, Atkins R, Greenhaff PL, Qi NR, Gustafsson T, Fielding RA, Timmons JA, Britton SL, Koch LG, Goodyear LJ.
Diabetes. 2013 Aug;62(8):2717-27. doi: 10.2337/db13-0062. Epub 2013 Apr 22.
23610057

BACKGROUND

SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

REFERENCES

Zhang, M., et al. J. Biol. Chem. 285(12):8703-8710(2010)
Daly, A.C., et al. J. Biol. Chem. 285(9):6489-6497(2010)
Heikkinen, P.T., et al. J. Biol. Chem. 285(6):3740-3749(2010)
Tseng, Z.H., et al. Heart Rhythm 6(12):1745-1750(2009)

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