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KRT1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - KRT1 Antibody (Center) AP9695c
    Western blot analysis of KRT1 Antibody (Center) (Cat. #AP9695c) in MCF-7 cell line lysates (35ug/lane). KRT1 (arrow) was detected using the purified Pab.
  • 14 - KRT1 Antibody (Center) AP9695c
    KRT1 Antibody (Center) (Cat. #AP9695c) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - KRT1 Antibody (Center) AP9695c
    KRT1 Antibody (Center) (Cat. #AP9695c) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P04264
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 3848
Other Names Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, CK-1, Hair alpha protein, Keratin-1, K1, Type-II keratin Kb1, KRT1, KRTA
Target/Specificity This KRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-443 amino acids from the Central region of human KRT1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKRT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT1
Synonyms KRTA
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Cellular Location Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells
Tissue Location The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis
Research Areas
Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor.
Author : Liu Y1,Zhong L2,Liu D1,Ye H1,Mao Y1,Hu Y1.
Mol Med Rep. 2017 Sep 8. doi: 10.3892/mmr.2017.7447. [Epub ahead of print]
28901412

BACKGROUND

KRT1 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

REFERENCES

Labudova, M., et al. J. Virol. 83(16):7842-7849(2009)
Barcelos, A.C., et al. J. Cutan. Pathol. 36(6):647-654(2009)
Grimberg, G., et al. Br. J. Dermatol. 160(2):446-449(2009)

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