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COXI Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 3 - COXI Antibody (Center) AP9684C
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling COXI with AP9684C at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing endoplasmic reticulum staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 3 - COXI Antibody (Center) AP9684C
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling COXI with AP9684C at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and mitochondrion staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
  • 1 - COXI Antibody (Center) AP9684C
    All lanes : Anti-COXI Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession P00395
Other Accession P00398, P05503, O79429, O79876, P00397, Q9MIY8, P18943, P00396
Reactivity Human
Predicted Mouse, Rat, Rabbit, Zebrafish, Pig, Chicken, Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4512
Other Names Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I, MT-CO1, COI, COXI, MTCO1
Target/Specificity This COXI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the Central region of human COXI.
Dilution IF~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOXI Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MT-CO1
Synonyms COI, COXI, MTCO1
Function Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
Cellular Location Mitochondrion inner membrane; Multi-pass membrane protein
Research Areas

REFERENCES

# Andrews, R.M., et al. Nat. Genet. 23 (2), 147 (1999) # Anderson, S., et al. Nature 290(5806):457-465(1981)

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